Westerns on uninfected H9 cells

[Mr. Goodbar]

brett wrote:

> glen, since when are negative controls useless?!?
> 
> Brett Lindenbach

	Negative controls are not useless,  the kit comes with a 
strong ab+, weak ab+ and an ab-.  Due to performance of the 
current assay, you won't see any H9 reactivity.  It would be like 
running an uncoated well in an ELISA.  There may be non-specific 
binding in the well, but the postcoating process would eliminate 
that.  Therefore, no need.



--
-----BEGIN PGP PUBLIC KEY BLOCK-----
Version: 2.6.2

mQBtAy90qe8AAAEDAMdtV33rZ5LAzn18s6ED5YvC+klj32kkTP9V5QFjwpVZCTHV
zdRJsQB7E3IdaamGT2GAo8zHc5y4e6NbDSvmCyBjjjBfIJuMUSp76DV9zds2K4Nn
z4GV9DeKHKJsypY8vQAFEbQuR2xlbiBBLiBQYW5uaWNrZSA8Z2xlbmFsYW5AbWFy
cy5zdXBlcmxpbmsubmV0Pg==
=PLkW
-----END PGP PUBLIC KEY BLOCK-----

pub   768/CA963CBD 1995/03/25 Glen A. Pannicke 
<glenalan at mars.superlink.net>
Key fingerprint =  6A 8E CF 4D D0 E3 61 DD  46 70 02 12 13 F9 12 
B7