Westerns on uninfected H9 cells

[brett]

>Todd Miller - Pharmacology wrote:
>> 
>> Looking over the pamphlet that comes with the Cambridge
>> Biotech Corp's Western blot kit for HIV detection, I
>> noticed there was no control strip with extracts from
>> uninfected H9 cells (the cells they claim they use to
>> propagate HIV for preparation of the "infected" strips).
>
>Todd,
>
>        H9 strips used to be used years ago, but with the specificity and
>sensitivity of 
>the current assay they are all but useless.  My company distributes CBC
>product.  Nice assay.
>
>Glen Pannicke
>


glen, since when are negative controls useless?!?



Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain