20 Kb insert

[Ron Goto x2832]

In article <Pine.SOL.3.91.951117103602.6888A-100000 at asrr.arsusda.gov> skleiboe at ASRR.ARSUSDA.GOV writes:
>Path: coh.Coh.ORG!news.cerf.net!usc!elroy.jpl.nasa.gov!swrinde!gatech2!sdd.hp.com!spool.mu.edu!olivea!biosci!ASRR.ARSUSDA.GOV!skleiboe
>From: skleiboe at ASRR.ARSUSDA.GOV
>Newsgroups: bionet.virology
>Subject: 20 Kb insert
>Date: 17 Nov 1995 07:40:04 -0800
>Organization: BIOSCI International Newsgroups for Molecular Biology
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>We are looking for a cosmid (or plasmid) that will accept a 18 - 22 Kb 
>insert.  We have looked at many catalogs etc. without finding an 
>appropriate vector.  If you have or know of someone who has a useful 
>vector please reply to:
>skleiboeker at asrr.arsusda.gov

>Thanks in advance,

>Steve Kleiboeker

Steve

We have a number of genomic lambda clones which  have been subcloned into 
bluescript, most are 15 kb or smaller but we have 6 clones larger. Two of 
these are 18-19 kb, they do not seem to grow any slower than my 'average' 
clone. In fact the slowest growing of my clones only has 12-13 kb insert. So I 
think bluescript can 'handle' 20-22 kb insert. (probably most of others can 
too). Just what we have done.
Good Luck

Ronald