GST and His tagged vectors

[Steven_H Harwood]

Does anyone have experience with using combination histidine and GSH 
transferase fusion vectors for expression and  sequential affinity 
purification of the resulting fusion proteins through Ni and GSH agarose 
resins?  There are several such vectors on the market (i.e. Pharmingen), 
but they claim their purpose in marketing them is for "customer 
flexibility" rather than for providing a dual affinity purification 
method.  Has anyone tried to purify an expressed fusion protein using 
both affinity methods?  Any responses would be appreciated.

Steve Harwood
Dept. Agricultural Chemistry
Oregon State University
Corvallis, OR 97331