(Fwd) Re infectivity of naked viral DNA

[DANDERSON at PRL.PULMONARY.UBC.CA]

ijiwaru at nyc.pipeline.com (Lyle Najita) wrote:
>Actually, the original Nature paper that described initiation of poliovirus
>replication from a cDNA clone was done from a pBR-type plasmid with no
>eukaryotic promoter element. They got lucky and the vector provided a
>reasonable facsimile. 
 
Interesting!  The plasmid containing the infectious cDNA of 
coxsackievirus B3 that was transfected into HeLa cells was also a 
pBR-type plasmid!  Of course most of todays plasmids are 
improved plasmids of the original pBR plasmid.

Dan