Virus aggregation advice

[Peter Young]

Keith
Totally agree with your views on the hype and paranoia re the Ebola 
outbreak, there are lots of dedicated people putting in a huge effort on 
this one, lets give them some support!

Re your questions on BVDV,
All batches of FCS in Australia will have both BVD virus and antibody. We 
have found that even small quantities of neutralising antibody in FCS 
will reduce the titre of cytopathic BVDV. You will therefore need to 
locate a source of serum which is free of BVDV  eg horse serum or serum 
from a bovine which is free of persistent infection and also free of BVDV 
antibody. Many veterinary diagnostic labs can do these tests.

Another important factor is that bovine testis cells (BT) are not 
uniformly susceptible. You need to use them at low passage eg <5 for best 
results.  They should also be shown to be free of persistent infection, 
and also therefore need to be grown in virus free serum (not FCS).

For more detailed advice contact Peter Kirklnad at the Elizabeth Macarthur 
Agricultural Institute, Camden, Sydney phone 046 293331 fax 293429
---------------------------------------------|-------------------------------
Peter Young, Veterinary Virologist           |phone:  07 3629578
Animal Research Institute and                |fax:    07 3629457
Queensland Agricultural Biotechnology Centre |e-mail: youngp at dpi.qld.gov.au
Brisbane, Australia.                         |
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On 12 May 1995, Keith Stuckly wrote:

> With all the hype and paranoia going on at the moment about the Ebola
...................(cut)
> 3. I am desperately trying to grow the Pestivirus Bovine Viral Diarrheoa
> Virus (BVDV) to high titre with limited success. I am currently using NADL
>.................(cut) 
> 
> Keith Stuckly    
> 
> 
>