Detection of retroviral infections: PCR vs RT-PCR
Brad Chadwick
chadwick at numbat.murdoch.edu.au
Thu May 11 15:34:47 EST 1995
Looking for some advice from anyone experienced in the relative
sensitivities of detecting low-level lentiviral infections such as HIV or
EIAV by standard PCR (essentially detecting provirus) compared to RT-PCR
should detect provirus and genomic transcripts)? Does RT-PCR tend to pick up
cases that standard PCR fails to detect from templates such as:
a)buffy coat
b)plasma or
c)any other tissue, such as archival paraffin-embedded stuff
Any references to same? Much obliged.
Brad C.
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