Agarose Gels

[MSLADE at RNA.BIO.MQ.EDU.AU]

>To:            virology at net.bio.net
>From:          mowjmat at coop.crn.org (Biology Department)
>Subject:       Agarose Gels
>Date:          25 Apr 1995 13:38:49 -0700
>
>	Recently, our gels have been running slanted--if you view the gel 
>from the side the bands are diagonal to the horizontal plane of the gel.  
>We have remade our buffer (1XTAE) but this does not seem to help.  Any 
>suggestions as to the cause of this problem?  The major difficulty is 
>that if you view the gels from above the bands appear fuzzy and wide 
>instead of sharp. Any comments would be appreciated.
>Thanks
>Jeff Huckaby
>Molecular Biology
>William Jewell College
>Liberty, Mo 64068
>email mowjmat at coop.crn.org
>------------------------------------------------------

I've just found the reference you  want:  Kozulic, B. (1994) Looking at bands from 
the other side. Anal. Biochem. 216, 253-261.  He identified five reasons for bent 
bands and how to overcome them.

Cheers, Martin Slade
Martin Slade,
School of Biological Sciences,
Macquarie University,
NSW 2109,
Australia
FAX  (61 2) 850 8174
Phone(61 2) 850 8210