Adenovirus assay

[Ian A. York]

In article <3obro0$cb5 at dingo.cc.uq.oz.au> mikep at biosci.uq.oz.au writes:
>I have been contracted to do some assays on some adenovirus, a virus I have
>never worked with before.  I have been told that I can assay by simple TCID50 on
>Vero cells.

	It's been a while since I worked with adeno.  Is this one of the
standard human adeonviruses - serotype 2 or 5?  If so, you might look for
reviews by Frank Graham.  You can do TCID50 - Vero cells certainly support
adeno with no problem.  I think you can also do plaque assays.  Often
people use the 293 cell line for growing adeno 5; 293 contain the E1
region from Ad and titres are better.  You might also support growth of
defective virus this way, but that doesn't seem to be a major problem. 
Titers of Ad5 are often in the high 10^9 to the low 10^10 per ml; however,
10^8 is not unusual.  Again, Ad5 is most often grown in 293 for making
preps. When I worked with a less cooperative adeno (bovine type 3) I 
ended up growing cells on microcarriers in suspension in order to get 
adequate titres.  

Ian





-- 
Ian York   (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921     Fax  (617)-632-2627