RNA purification

[Francisco Muril Zerbini]

On Thu, 4 May 1995, Mike Poidinger wrote:

> I have purified some Alphavirus RNA for cDNA library synthesis only to
> find it contaminated with cellular RNA, mostly t and r. Does anyone
> have a good technique for size fractionation of the RNA such that the
> viral RNA will remain intact and useable in forst strand cDNA synthesis. 

How about running it through an oligo-dT column ??

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| Murilo Zerbini                   | Out of 3,000,000,000 DNA nucleotides, |
| Dep. of Plant Pathology          | human beings and chimpanzees have     |
| University of California, Davis  | 2,999,400,000 in common.              |
| fmzerbini at ucdavis.edu            |                                       | 
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