RNA purification

[Francisco Muril Zerbini]

On Thu, 4 May 1995, Mike Poidinger wrote:
> I have purified some Alphavirus RNA for cDNA library synthesis only to find it
> contaminated with cellular RNA, mostly t and r.
> Does anyone have a good technique for size fractionation of the RNA such that
> the viral RNA will remain intact and useable in forst strand cDNA synthesis.

OOOOPS !!! I'm just thinking here, alphavirus RNA is not polyadenylated, 
is it ? If it is not, then forgot the oligo-dT columns ! Sorry, I work 
with potyviruses...

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| Murilo Zerbini                   | Out of 3,000,000,000 DNA nucleotides, |
| Dep. of Plant Pathology          | human beings and chimpanzees have     |
| University of California, Davis  | 2,999,400,000 in common.              |
| fmzerbini at ucdavis.edu            |                                       | 
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