Case of the Rearranging Plasmid?

[Ian A. York]

In article <3o6s77$p4r at sifon.cc.mcgill.ca> Graham Dellaire <popa0206 at PO-Box.McGill.CA> writes:
>I have prepared a plasmid based vector that contains a large terminal fragment of
>the adenovirus-2 genome.  The vector is 22 kbp and has the odd and frustrating
>ability to recombine and rearrange when grown in bacteria in large culture volumes

I haven't done this myself, but I used to work up the hall from Frank
Graham and his lab and I seem to recall that the terminal fragments of Ad
were sometimes odd in bacteria.  I'm afraid I don't remember the details,
but I have a feeling that one of Frank's reviews may have referred to
this, so you might run through them.  Not much help, I'm afraid. 

Ian

-- 
Ian York   (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921     Fax  (617)-632-2627