HIV sequence from virus

[A.J.Cann]

tmiller at newssun.med.miami.edu (Todd Miller - Pharmacology) wrote:
>In article <3r7bdh$gc9 at ipgate.le.ac.uk>, A.J.Cann <nna at le.ac.uk> wrote:
>>Many HIV RNA sequences have been derived by limit-dilution RT-PCR of 
>>virus particles. They do show significant differences from the prevalent 
>>(DNA) provirus sequences at any one time. Check out the may references 
>>by A.Leigh-Brown & P.Simmonds, Edinburgh University.
>>
>I looked at the most recent article in January J Inf Diseases and
>was referred back to a 1990 paper by the same authors for methods.
>All the methods used proviral DNA.

I suggest you go & read a few of their other papers until you find the ones 
that refer to RNA.

>No more wild goose chases for me.  I'm convinced that there is no
>sequence data derived from particles, and when this is considered
>along with the absence of EM's of pure HIV, I have to doubt it's
>existence.  Why isn't this data available?  All you thousands of
>scientists working on this and no one can provide me this info.

I just did.