Help! Questions about transcriptional elements in SV40 'control' region
A. M. Lotto
aurelia at u.washington.edu
Thu Jun 1 19:51:31 EST 1995
Hello,
I am in desperate need of an explanation of some transcriptional
elements of SV40.
<-- early tata 21 bp repeats 72 bp segments
____________________________________________________________________________
| XXXX | 21 | 21 | 21 | | 72 | 72 |
----------------------------------------------------------------------------
Ori late -->
My questions regard the sequence of events. Enhancers bind to TFIID.
SP1 binds to GC boxes in 21 bp repeat sequences. TFIID interacts with
SP1 and SP1 interacts with RNA Pol II which doesn't know where to bind
the DNA. First, I am not entirely sure the previous sequence of events
is even close to correct, secondly, how do these components interact to
increase the efficiency of txn? Where does TFIID bind? My sources say
that the enhancers bind to the 72 bp sequences but also that TFIID binds
enhancers. I am confused about this and would greatly appreciate anyone
able to shed some light on these interactions.
Thanks very much.
Amy Lotto
aurelia at u.washington.edu
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