virus purification needed
WAS
SHOREWA at UCBEH.SAN.UC.EDU
Mon Feb 27 09:00:25 EST 1995
I have been assigned a task to isolate the DNA from a circovrus. I have
tried a few protocols but nothing has seemed to work. I would like some
information on DNA isolation techniques for ssDNA and possibly dsDNA from
virus. Basically what I have done so far is to isolate the avian genomic
DNA in hopes that the smaller 2kb viral genome comes with it. I have had
no success as yet. I posted this query to molbio.methods-reagents and
came up with a Hirt extraction. Haven't tries the Hirt yet, but will
this morning. Another question I have is what would be the difference
between Proteinase K and Pronase ?
Thanks for any help
Alan Shore Univ. of Cincinnati
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