polybrene question

[Chuckles]

Patrick O'Neil <patrick at corona> writes:

>On 27 Jan 1995, Chuckles wrote:

>> The theory that I have heard is that polybrene coats membranes and thus shields
>> the negative charges on the surface.  This allows membranes to come together
>> more easily and facilitates viral fusion. 

>If this is the case, would not PEG also serve (in as much as it inhances 
>membrane fusion), or just ethlylene glycol?

I don't know.  Has anybody every tried adding these to the mix and seeing what happens?
All I know is that polybrene does increase titer and XC cell fusion, and what I wrote above
is the best explination I have heard concerning why it works.

					In search of better fusion everywhere,

					Chuckles