"LONG" RT/PCR (fwd)

[Dr Michael J Carter]

> 
> To:            virology at net.bio.net
>           
> Subject:       Re: "LONG" RT/PCR
> Date:          16 Nov 1994 15:05:21 GMT
> 
> 
> 
> 
> I have also found that the RT reaction is limiting.  Typically I do RT 
> extensions for 1 1/2 hrs.  I have also found that the primers can be 
> limiting so adding additional excess 3' primers to the RT or PCR reactions 
> can increase the amount of products 
> 
> JV
> 
Dear JV,

How excess is excess?

Ta

Mike Carter> 
>