To: virology at net.bio.net
Subject: Re: "LONG" RT/PCR
Date: 16 Nov 1994 15:05:21 GMT
I have also found that the RT reaction is limiting. Typically I do RT
extensions for 1 1/2 hrs. I have also found that the primers can be
limiting so adding additional excess 3' primers to the RT or PCR reactions
can increase the amount of products
JV