"LONG" RT/PCR

[Steve Wylie]

Has anyone tried RT/PCR for RNA of 10KB or longer? I have tried to do 
this with the potyvirus (BYMV) I work with, but so far no success. 4Kb 
isn't a problem but anything longer is! I suspect the RT to be the 
limiting step so have tried M-MLV and AMV RT's, incubating for 3-4 hours 
but still nothing. Any comments will be gratefully accepted.
Steve Wylie, Murdoch University, Western Australia