HSV-2 pool

[M K Bennett]

Angelo J. Pinto (PINTO at CCC.MEDCOLPA.EDU) wrote:
: Can anyone give me any helpful suggestions on how to grow a 
: high-titered HSV-2 pool that is pathogenic in mice?

This may be too obvious, but why dont you grow up the virus in cell
culture?  I work with HSV-2 MS and grow up my stocks in Vero cells and can
get titres around 1-100 million pfu/ml.

mike

-----------------------------------------------------------------------------
Mike Bennett D.Phil		|Scientists have odious manners, except when
Dept Pathology and Microbiology,|you prop up their theory; then you can borrow
University of Bristol, UK	|money from them--Mark Twain 1917 
m.k.bennett at bristol.ac.uk	|
-----------------------------------------------------------------------------