potyvirus purification

Steve Wylie wylie at CSUVAX1.MURDOCH.EDU.AU
Mon Jul 25 20:25:09 EST 1994

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Does anyone know of an efficient method for purifying potyviruses from 
plant material? I have been using the method of Hammond and Lawson (1988) 
which works but results in quite low yields. This method utilises PEG 
8000 to precipitate the virus particles then a CsCl or Cs2SO4 gradient to 
purify them. There are several methods available but with limited plant 
material and time I can't try them all. Any comments appreciated.
Steve Wylie, Murdoch University, Western Australia

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