virus particles

[Ted van der Lende]

Dear virologists

I'm a Dutch PhD student working on a virus disease of the common 
mushroom (Agaricus bisporus). At the moment we're trying to develop
a RNA polymerase assay for the isolated virus particle. For this 
purpose we want to disrupt the isometric virus particle without 
denaturing the  viral proteins. In other words we want to avoid the 
use of detergents, high salts etc. Does anyone have any experience on 
this subject? 

Ted van der Lende 
Department of Plant Biology
University of Groningen
Kerklaan 30 
9751 NN Haren
The Netherlands
email: LENDETR at biol.rug.nl