Flu virus

[lxia at COR-MAIL.BIOCHEM.HMC.PSU.EDU]

Dear netters:
I am currently conducting an experiment which will use flu viruses
(A/WSN/33). The first thing I had done was making virus stocks.
Unfortunately, I was unable to make a high titer stock by using MDBK cells.
Could someone out there give me some ideas about the proper way to make the
flu virus stocks.
Thanks a lot.
Liang Xia
LXIA at COR-MAIL.BIOCHEM.HMC.PSU.EDU
HERSHEY MEDICAL CENTER
PENN STATE UNIVERSITY
HERSHEY, PA 17033