MAPseq and BARseq

Reviews

• Barcode-based connectomics (concept + limitations summary, 2024) — short overview contrasting MAPseq, BRICseq, BARseq, and ConnectID, incl. tradeoffs for adding transcriptomes. eLife
• From MAPseq to BRICseq and beyond (2021) — narrative review of barcoded projection mapping from the Zador lab lineage. PMC
• Neural circuit research with molecular barcodes (2025) — broader survey placing MAPseq/BARseq among barcoded neurotech. ScienceDirect

MAPseq — essentials

• MAPseq (Neuron, 2016) — seminal method: infect neurons with a diverse RNA-barcode virus; bulk-sequence dissected target regions to read one-to-many projections at scale. (Open access.) PMC
• MAPseq (primary index / PubMed) — canonical reference & metadata. PubMed
• MAPseq2 (preprint, 2025) — protocol update reporting \~3–4× higher barcode detection sensitivity and \~10× lower cost vs. original MAPseq. ResearchGate

See also

See in situ sequencing for more about BARseq, BARseq2, etc.

See lineage tracing for other cell tracing and cell lineage tracing techniques.

One interesting alternative is compressive sensing like "Rapid learning of neural circuitry from holographic ensemble stimulation enabled by model-based compressed sensing" or this ref00224-6).