Microfluidic equipment relevant to homebrew biology, diybio, biohacking, etc. # centrifugation * DVD mount, run in DVD player * food blender with custom chuck to hold slide # electroporation Sonoporation is probably the most simple for microfluidic designs. # separation * "Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channels" * sound: * * "Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW)" * * "Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays" * * "Chip integrated strategies for acoustic separation and manipulation of cells and particles" * tangential flow filtration ## DNA separation * pillar/post/peg array * * Sometimes with an electric field * * * "A DNA prism for high-speed continuous fractionation of large DNA molecules" * * * "Separation of long DNA molecules by quartz nanopillar chips under a direct current electric field" * jagged channel edge (see "Size-dependent free solution DNA electrophoresis in structured microfluidic systems") * electrophoresis * * "Electrophoretic separations on microfluidic chips" * * capillary electrophoresis * * capillary gel electrophoresis (CGE) * * microchip-based capillary electrophoresis * * pulsed-field gel electrophoresis (PFGE) * * capillary zone electrophoresis * * micelle electrokinetic chromatography (MEKC) * * isoelectric focusing (IEF) * * * isoelectric focusing also works for protein separation "Concentration and separation of proteins in microfluidic channels on the basis of transverse IEF" ## chromatography The efficiency of chromatography increases with column length. Electrophoresis is often used in microfluidics instead of chromatography because "glass and plastics cannot withstand the pressures that would be needed; it is difficult to pack the channels without voids there are mass transfer problems in the corners of microchannels; voids cause unacceptable band broadening". * hydrodynamic chromatography * size exclusion chromatography * "High-aspect-ratio, silicon oxide-enclosed pillar structures in microfluidic liquid chromatography" * "Liquid phase chromatography on microchips" * "Fabrication of high-quality microfluidic solid-phase chromatography columns" * "Application of monoliths for bioparticle isolation" ## fractionation * digital isoelectric fractionation (using separate pH-specific membranes based on isoelectric points of analytes) * field flow fractionation * nanochannels with step-size changes corresponding to lengths of particles to separate # PCR yep... many methods. # DNA sequencing not yet # DNA synthesis nope # cell culture sure # pipettes (just kidding.. use a regular pipette?) # thermometer isn't this already microfluidic? or is it millifluidic?