## Electrophoresis Gel Box Power Supply
* [Building a cheap power supply](http://wiki.biohackers.la/Cheap_Power_Supply)
* See Open Gel Box 2.0 Power Supply on OpenWetWare. Working unit has been constructed.
## 3D Printed Gel Boat and Comb Set
To: DIYBio google group
From: Cathal
Date: Sep 17, 10:07 am
I've just published my first designs on Shapeways, for a $100 Gel Boat
and Comb set.
Sit these into a plastic container with some graphite electrodes at
either end, and you have a complete gel electrophoresis kit.
I intend to make more designs and share them both on this shop and
with the open-source community in the near future!
http://www.shapeways.com/shops/labsfromfabs
The designs are open source, and are available on Thingiverse.com for
those with access to a 3D printer.. just be sure and let me know the
results please! I'm awaiting my own 3D printer right now so I'm
itching to see results.
Note: Because of the opaque nature of the printed plastic, UV
visualisation will have to be Top-down.
## Gel Box from Tupperware or other
* THE MACGYVER PROJECT: GENOMIC DNA EXTRACTION AND GEL ELECTROPHORESIS EXPERIMENTS USING EVERYDAY MATERIALS. By Yas Shirazu, Donna Lee, and Esther Abd-Elmessih. DNA extraction and separation by agarose gel electrophoresis is a simple and exciting process that anyone can perform. However, the high cost of specialized equipment and chemicals often hinder such an experiment from being carried by members of the high school community. Here, we describe a cost effective way of extracting and electrophoresing DNA under a prescribed MacGyver limitation – that is using only materials available from a grocery store or shopping mall.
* ''add your gel box project here''
# Inkjet Printer Hacking for Bio Projects
Many papers over the years involve using either EPSON inkjet printers (piezoelectric print head) or HP/etc inkjet printers (thermal print head) to dispense very small amounts of biomaterial or biochemicals.
## EPSON Printers
Epson is the only brand which uses piezoelectric print heads. Epson printers also have CD-printing, which provides a mechanical method to feed larger objects into the printer.
* Epson R280
** Open Access research paper: "This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was adapted for chemical printing; E. coli cultures were grown on 750 µm thick agar embedded in micro-wells machined into commercial compact discs. Spatio-temporal regulation of the lac operon was demonstrated via the printing of patterns of lactose and glucose directly into the cultures; X-Gal blue patterns were used for visual feedback. We demonstrate how the bistable nature of the lac operon's feedback, when perturbed by patterning lactose (inducer) and glucose (inhibitor), can lead to coordination of cell expression patterns across a field in ways that mimic motifs seen in developmental biology."
:: Cohen DJ, Morfino RC, Maharbiz MM, 2009 ''A Modified Consumer Inkjet for Spatiotemporal Control of Gene Expression.'' PLoS ONE 4(9): e7086. doi:10.1371/journal.pone.0007086
:: As discussed on the DIYbio google group:
## HP, Canon, .... Printers
* ''Add inkjet printer projects here''
## BioCurious printer
*