Pulling secondary-list indents back to four spaces seems to work.HEADmaster

1 files changed, 17 insertions, 17 deletions

diff --git a/E.coli Transformation With PEG+MgSO4.md b/E.coli Transformation With PEG+MgSO4.md
index 00e968b..b87a859 100644
--- a/E.coli Transformation With PEG+MgSO4.md
+++ b/E.coli Transformation With PEG+MgSO4.md
@@ -11,33 +11,33 @@ The following protocol is designed to make it as easy for a beginner to make the
 * Optional centrifuge
 * Optional autoclave/pressure cooker
 
-## Minimum equipment;
-* Sterile Glass or Pasteur Pipettes or glass droppers, preferably graduated.  
-    - If using glass pipettes, you'll also need a pipette pump and a pipette brush, available cheaply on ebay.  
-    - Glass droppers must be sterilisable by pressure cooker.  
+## Minimum equipment
+* Sterile Glass or Pasteur Pipettes or glass droppers, preferably graduated.
+    - If using glass pipettes, you'll also need a pipette pump and a pipette brush, available cheaply on ebay.
+    - Glass droppers must be sterilisable by pressure cooker.
     - Disposible pasteur pipettes should be graduated and sterile, pre-wrapped.
 * 30C incubator - achievable with a pet terrarium thermostat and heat-mat in a polystyrene box.
 * Crushed ice
 * Microwave for sterilising media (pressure cooker preferred!)
-* Sterile work area;  
-    - Bunsen burner or blowtorch-facing-up, or  
-    - Jury-rigged glove-box with inner surface sterilised, or  
+* Sterile work area
+    - Bunsen burner or blowtorch-facing-up, or
+    - Jury-rigged glove-box with inner surface sterilised, or
     - A good HEPA-rated air purifier with a fresh filter pointing at work area.
 * Lab strain, plasmid-free E.coli
 * Desired DNA plasmid
-* Ingredients for basic broth:  
-    - Soy Protein (or lactose-free casein)  
-    - Yeast Extract with no added salt (or, balance salt content against your added salt below)  
-    - Salt, non-iodised, preferably without preservatives  
+* Ingredients for basic broth:
+    - Soy Protein (or lactose-free casein)
+    - Yeast Extract with no added salt (or, balance salt content against your added salt below)
+    - Salt, non-iodised, preferably without preservatives
     - Bromelain or Papain digestive tablets, or Meat tenderiser
-* Additional ingredients for TSS-broth:  
-    - PEG-3350, sold under a number of brand names as a laxative: Miralax, GoLytely, GlycoLax, etc. Probably available through pharmacy over the counter, or internet.  
+* Additional ingredients for TSS-broth:
+    - PEG-3350, sold under a number of brand names as a laxative: Miralax, GoLytely, GlycoLax, etc. Probably available through pharmacy over the counter, or internet.
     - MgSO4, sold as “Epsom Salt” in pharmacies as a bath salt.
 * Fine Sieve, Coffee filters, funnel, cotton wool to clarify broth
-* Glassware  
-    - Preferably actual lab glassware, available from brouwland.com or various ebay sellers. Alternatively;  
-    - Recycled jam jars for “Flasks”  
-    - Smaller jars for “test tubes”  
+* Glassware
+    - Preferably actual lab glassware, available from brouwland.com or various ebay sellers. Alternatively;
+    - Recycled jam jars for “Flasks”
+    - Smaller jars for “test tubes”
     - Wide jars or glass ramekins for “Petri Dishes”.
 
 ## Transforming E.coli by a modified TSS method: